Primer

Part:BBa_K300975

Designed by: Manuel Lupotto, Matteo Meroso, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-21)

Phi80 locus forward primer

This primer (in house known as P1) anneals in the genomic region flanking the attB(Phi80) site and can be used together with BBa_K300978 to amplify the entire Phi80 locus. It can also be used together with BBa_K300976 (which anneals on BBa_K300000 integrative base vector for E. coli) to verify the correct integration of the part of interest (through BBa_K300000) in the Phi80 locus of the E. coli genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None